The present invention relates to a method of preparing a mesoporous carbon material with layered structure using humin. The preparation method provided by the present invention may prepare a mesoporous carbon with layered structure, especially a mesoporous carbon with layered structure having a regular frame. Prepared by the present invention, the mesoporous carbon with layered structure having the regular frame has a specific surface area up to 1000 m/g and an average pore diameter of 3 nm-6 nm